Petri dishes having media are incubated in inverted position after inoculation. This has different advantages those improve the result accuracy. Ankur Choudhary Print Question Forum 5 comments. Petri dishes were first used by German physician Julius Petri in He covered one culture plate to another plate to prevent the contamination.
Before him, the culture plates were covered with the glass bell jars. All of we know that the Petriplates are incubated in inverted position but this question arises in our mind that why these plates are incubated in inverted position?
I got many questions daily from pharmaceutical professionals and this question is asked very frequently. There are following advantages during incubation when we incubate the plates in inverted position. When the plates are incubated in the normal position, water evaporation from media occurs. These vapors condense on the lid of the Petri dish and drops fall on the colonies developed on the media surface. This causes colony mix-up with each other and spread throughout the plate surface.
This creates the problem in counting and proper determination of microbial count. Maintain it at an angle at all timese. In a test tube rack at all timesQuestion 2Not yet answered Marked out of 1. The best definition of a pure culture is A culture with pure types of different bacteria, A culture with only one type of bacteria. A culture with different layers of bacteria to be isolated. A culture that is not contaminated. Which of the following What is a major difference between the quadrant streak and zigzag inoculation method?
Using a sterile instrument for streaking after the initial streakb. Identifying a mixed culturec. Amount of space of plate the experiment usesd. Exercise 8 Questions 1. Define a culture medium. A culture media are agar gelatinous or liquid medium nutritive substances such as microorganisms, animal cells, plant cells, or tissues and are cultivated for scientific purposes. The American Create an Account and Get the Solution. Log into your existing Transtutors account.
Have an account already? Click here to Login. Identifying plague bacteria — During another outbreak of the plague in in Asia, a Swiss-French bacteriologist called Alexandre Yersin discovered that the Yersinia pestis bacterium was responsible for the condition. He also identified which culture media optimised the development of the bacillus, thereby allowing scientists to reproduce the same conditions in their laboratory and study the microorganism.
Discovery of Penicillin — Perhaps one of the most famous pharmaceutical discoveries that the Petri dish can be attributed to is that of Penicillin. Staphylococcus is the bacteria responsible for the likes of boils, sore throats and abscesses. Fleming noticed something unusual about one of his Petri dishes as it was dotted with colonies apart from in one area where a blob of mould was growing. The ePetri was designed to do away with the need for bulky microscopes and significantly reduce human labour time, while improving the way in which the culture growth can be recorded.
A small camera in the dish can send data from the ePetri dish and transfer it to a computer outside the incubator by a cable connection. This is said to save time and reduce contamination risks. Culturing brain tissue — Petri dishes successfully aided scientists at the University of Luxembourg in their attempts to turn human stem cells into brain-like cultures that behave very similarly to cells in the human midbrain.
The research was conducted in and involved stem cells, derived from human skin samples, being grown on Petri dishes. As the brain is the most complex human organ and research surrounding it faces many ethical implications, it can be extremely difficult to conduct experiments on it. Growing organs — In more recent years, regenerative medicine has advanced rapidly and the Petri dish has played a role in the growing of skin grafts and organoids.
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